A High-Resolution, Single-Grain, <em>In Vivo</em> Pollen Hydration Bioassay for <em>Arabidopsis thaliana</em>
نویسندگان
چکیده
Sexual reproduction in flowering plants requires initial interaction between the pollen grain and stigmatic surface, where a molecular dialog is established interacting partners. Studies across range of species have revealed that series checkpoints regulate pollen-stigma to ensure only compatible, generally intraspecific successful effecting fertilization. In possess 'dry stigma', such as model plant Arabidopsis thaliana, first post-pollination, prezygotic compatibility checkpoint establishment hydration. This phase pollination tightly regulated, whereby signals from elicit release water stigma, thus permitting The ability accurately measure track hydration over time key design experiments directed at understanding regulation this critical step reproduction. Published protocols frequently utilize flowers been excised parent plant, maintained on liquid or solid media, bulk pollinated. paper describes noninvasive, vivo bioassay permits minute-by-minute tracking individual A. thaliana grains high resolution. assay highly reproducible, able detect very subtle variations profiles, suitable for analysis mutants affect pathways regulating pollination. Although protocol lengthier than those described pollinations, precision reproducibility it provides, along with its nature, make ideal detailed dissection phenotypes.
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2023
ISSN: ['1940-087X']
DOI: https://doi.org/10.3791/65280